Journal: Marine Drugs

Article Title: Orally Administered Rhamnan Sulfate from Monostroma nitidum Significantly Inhibits Melanoma Metastasis in Lungs and Aorta of Mice Implanted with B16 Cells

doi: 10.3390/md24040126

Figure Lengend Snippet: ( A ) Changes in the expression of melanoma cell metastasis-related factors in the M + W group mice. The expression levels of melanoma cell metastasis-related factors in the blood and tissues of M + W group mice implanted with B16 cells were compared to those in the control group. Factors that significantly increased are indicated by upward arrows, factors that remained unchanged are indicated by horizontal dotted lines, and factors that significantly decreased are indicated by downward arrows. The combined effects of changes in the expression of each factor are thought to promote melanoma cell metastasis. ( B ) Changes in the expression of melanoma cell metastasis-related factors in the M + RS group mice. The expression levels of melanoma cell metastasis-related factors in the blood and tissues of the M + RS group mice implanted with B16 cells were compared to those in the M + W group. Factors that significantly increased are indicated by upward arrows, factors that remained unchanged are indicated by horizontal dotted lines, and factors that significantly decreased are indicated by downward arrows. The combined effects of changes in the expression of each factor are thought to suppress melanoma cell metastasis. Abbreviations: PAR, protease-activated receptor; TGFβ1, transforming growth factor β1; IL-6, interleukin 6; TF, tissue factor; TNFα, tumor necrosis factor α; F4/80, macrophage marker F4/80; Ly6G, lymphocyte antigen 6 complex locus G6D; PAD4, peptidylarginine deiminase 4; cisH3, citrullinated Histone H3; TM, thrombomodulin; uPA, urokinase-type plasminogen activator; TAFI, thrombin-activatable fibrinolysis inhibitor; TAFIa, activated TAFI; MMP, matrix metalloproteinase; Ang-2, angiopoietin-2; bFGF, basic fibroblast growth factor; Robo4, Roundabout homolog 4; E-cadherin, epithelial cadherin; Snail-1, small family zinc finger 1; Wnt, wingless and int-1; Wnt3a, Wnt family member 3a; LRP, low-density lipoprotein receptor-related protein; Frizzled, Frizzled class receptor.

Article Snippet: The levels of PAR1, PAR2, TF, TNF-α, TGF-β1, Ang-2, bFGF, β-catenin, vimentin, fibronectin, and Snail-1 in tissues were determined using commercially available ELISA kits: PAR1 (MBS753326, MyBioSource, San Diego, CA, USA), PAR2 (MBS4501658; MyBioSource), TF (ab214091; Abcam), TNF-α (KE10002, Proteintech), TGFβ1 (E-EL-M0051, Elabscience, Houston, TX, USA), Ang-2 (MANG20, R&D Systems), bFGF (bs-0217R, Bioss Antibodies), β-catenin (ADI-900-135; Enzo Life Sciences, Executive Blvd Farmingdale, NY, USA), fibronectin (OKCD05702, Aviva Systems Biology, San Diego, CA, USA), vimentin (ELK3731, ELK Biotechnology, Denver, CO, USA), and Snail-1 (LS-F2317-1, LS Bio, Shirley, MA, USA).

Techniques: Expressing, Control, Marker

Journal: Materials Today Bio

Article Title: Multidimensional nano-ion composite hydrogel based on enzymatic blood glucose control, gas therapy and ion liquid permeation for repairing diabetic wounds

doi: 10.1016/j.mtbio.2026.103213

Figure Lengend Snippet: Skin immunofluorescence staining and immunohistochemical analysis were performed in different treatment groups. A) Immunofluorescence staining images of NF200. β 3 -Tubulin. PGP9.5. CD31. VEGF and IL-6 in skin wounds on the 14th day after treatment. Scale = 400 μm. B) Immunohistochemical images of FGF2 on day 14 in wound tissue. Scale = 400 μm. C) Images of the immunohistochemistry of AGEs. Scale bar = 400 μm. D) Fluorescence intensity of NF200. E) Fluorescence intensity of β 3 Tubulin. F) Fluorescence intensity of PGP9.5. G) CD31-labeled micro vessel density. H) Fluorescence intensity of VEGF. I) Fluorescence intensity of IL-6. J) Integrated option density (IOD) of FGF2. K) IOD of AGEs. Data are represented as mean ± SD (n = 3). Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey's test ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. ∗∗∗∗ p < 0.0001.

Article Snippet: After routine deparaffinization, rehydration, and antigen retrieval, the sections were blocked and then incubated overnight at 4 °C with primary antibodies against FGF2 (1:500, GB113751 , Servicebio) and AGEs (1:200, bs-1158R, BIOSS).

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Immunohistochemistry, Fluorescence, Labeling

Journal: Bioactive Materials

Article Title: Bioinspired scaffold recapitulating chondrogenic ontogeny and microenvironment for functional cartilage regeneration

doi: 10.1016/j.bioactmat.2025.11.041

Figure Lengend Snippet: Spatiotemporal remodeling of FGF signaling networks in chondrocyte-like subpopulations. A) Heatmap of FGF signaling mediated communication patterns across chondrocyte-like subpopulations. B) Circle plot visualizing differential inter-subpopulation communication of FGF ligand-receptor signaling in chondrocyte-like cells at 4 week. C) Circle plot visualizing differential inter-subpopulation communication of FGF ligand-receptor signaling in chondrocyte-like cells at 8 week. D) Violin plots of expressions of FGF genes. E) Bubble plot of dysregulated ligand-receptor pairs. F) The differences in the overall information flow in the network. G) Representative western blots showing protein levels of FGF2 and FGF18 in Control and CE-SKP co-cultured cells.

Article Snippet: After blocking the membranes for an hour at room temperature using fast blocking solution, the membranes were incubated with primary antibodies specific for SOX9 (1:2000, 67439-1-Ig, Proteintech), COL2 (1:200, NBP1-91056, Novus Biologicals), COL10 (1:500, 26984-1-AP, Proteintech), COL1 (1:2000, 67288-1-Ig, Proteintech), RUNX2 (1:200, 20700-1-AP, Proteintech), FGF2 (1:500, 11234-1-AP, Proteintech), FGF18 (1:500, 60341-1-Ig, Proteintech) and GAPDH (1:50000, 60004-1-Ig, Proteintech) for an entire night at 4 °C.

Techniques: Western Blot, Control, Cell Culture